Molecular properties and extracellular processing of the lipase of Staphylococcus warneri M.
JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY(2012)
摘要
Staphylococcus warneri M exhibited extracellular lipase activity. By zymogram analysis of extracellular proteins, multiple bands were detected and the profiles changed depending on the bacterial growth phase. N-terminal amino acid sequences of three bands (N1-N3) were determined. From the genome library of S. wameri M whole DNA, the gene-directing lipase activity (named gehC(wm)) was cloned and characterized. The gehC(wm) gene encoded a protein (GehC(wm)), whose calculated molecular mass was 83.4 kDa, and the sequence was similar to the other staphylococcal lipases. Though two lipases have been known from S. warneri 863, GehC(wm) differs from both of them, indicating that this enzyme is the third extracellular lipase of the S. warner! strain. The N-terminal sequences of the N1-N3 polypeptides completely coincided with the deduced amino acid sequences in GehC(wm). GehC(wm) was predicted to be a prepro-protein. In vitro processing and protein sequencing suggested that pro-GehC(wm) is possibly processed by extracellular glutamyl endopeptidase, PROM. Inductively coupled plasma-atomic emission spectrometer analysis showed that purified histagged mature GehC(wm) possessed zinc ion. A gehC(wm) knockout mutant was constructed by insertion of an erythromycin resistance gene into the gehC(wm). Zymogram and immunoblot analyses of the gehC(wm) mutant indicated that GehC(wm) was a major extracellular lipase of S. warneri M. Copyright (C) 2012 S. Karger AG, Basel
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关键词
Lipolytic enzyme,Staphylococcus warneri,Processing,Glutamyl endopeptidase
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