Application Of Near Infrared Spectroscopy In Rapid And Simultaneous Determination Of Essential Components In Five Varieties Of Anti-Tuberculosis Tablets

Since 1980s, tuberculosis has become increasingly serious. Rifampicin tablets, isoniazide tablets, pyrazinamide tablets, rifampicin and isoniazide tablets and rifampicin isoniazide and pyrazinamide tablets are currently relatively efficacious antituberculosis drugs. In the present paper, near infrared spectroscopy (NIRS) with partial least squares (PLS) was applied to the simultaneous determination of rifampicin (RMP), isoniazide (INH) and pyrazinamide (PZA) contents in 5 varieties of anti-tuberculosis tablets. As the results showed, all of the models for the determination of RMP, INH and PZA contents applied the original NIR spectra. The most efficacious wavelength range for the determination of RMP contents was 1 981-2 195 rim, it was 1 540-1 717 rim and 2 086-2 197 nm for the determination of INH contents, and it was 1 460-1 537 nm, 1 956-2 022 run and 2 268-2 393 run for determination of PZA contents. The root mean square error of the calibration set obtained by cross-validation (RMSECV) of the optimum models for the quantitative analysis of RMP, INH and PZA contents was 0.049 4, 0.025 7 and 0.030 7, respectively. Using these optimum models for the determination of RMP, INH and PZA contents in prediction set, the root mean square error of prediction set (RMSEP) was 0.018 2, 0.016 6 and 0.013 4, respectively. The correlation coefficient (r(p)) between the predicted values and actual values was 0.986 4, 0.998 9 and 0.999 3, respectively. These results demonstrated that this method was precise and reliable, and is significative for in situ measurement and the on-line quality control for anti-tuberculosis tablets production.