Femtomolar Fab Binding Affinities to a Protein Target by Alternative CDR Residue Co-Optimization Strategies Without Phage or Cell Surface Display.
MAbs（2012）
Key words
compartmentalized screening,directed evolution,affinity maturation,antibody,protein-protein interactions,yeast display,phage display,saturation mutagenesis,in vitro recombination,high throughput screening,HTS ELISA assays,HTS TR-FRET assays,adalimumab,humira,femtomolar
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