[Human hR24L gene is involved in DNA excision repair and recombination repair].

OBJECTIVE:To study the correction phenotype of temperature sensitive mutant rad24LR of S. cerevisiae and to clone wild human functional homologue. METHODS:The temperature sensitive mutant rad24LR was studied by complementation test. Transformation of rad24LR was performed with human cDNA expression library to screen for reversion phenotype. RESULTS:RAD24L can complement the radiation sensitive phenotype of the mutation. Its homologue of human cDNA was cloned by screening transformed rad24LR with human expression library for reversion phenotype. CONCLUSION:Human DNA repair gene hR24L was successfully cloned in this study. The product hR24L is involved in both DNA excision repair and recombination repair.