Granulocyte colony stimulating factor (GCSF) protected in ovarian tissues against ischemia-reperfusion injury

Ovarian torsion is an important reproductive gynecological emergency in daily life and physiology. Granulocyte colony stimulating factor (G-CSF) is a glycoprotein capable of hematopoietic development and successfully used in the treatment of congenital granulocytopenia. In our study, we aimed to investigate protective effects of G-CSF on ovarian ischemia reperfusion (IR) immunohistochemically. Four groups were used. In control group, abdomen was opened and closed with the surgical protocol. The rats in the G-CSF group were given 100 µg/kg G-CSF subcutaneously before the IR and the abdomen was surgically opened and closed. 3-h of ischemia was and then 3-h reperfusion was induced in the ischemia-reperfusion (I/R) group. In the IR+G-CSF group, 100 µg/kg G-CSF was given before the procedure and IR was performed. At the end of the experiment, ovarian tissues were fixed in 10% formalin and then processed for routine paraffin tissue protocol. Normal ovarian histology was observed in the control and G-CSF groups. In the IR group, vascular dilatations, hemorrhage and increased inflammation were observed. In the I/R+G-CSF group, pathology in seen IR was decreased. IL-6 expression was mainly negative in control and G-CSF groups. Positive IL-6 immune reaction was observed in the granulosa cells and stromal area. In the I/R+G-CSF group, IL-6 expression was significantly decreased in ovarian follicular structures and in the stromal area compared to the I/R group. In conclusion, G-CSF reduced vascular dilatation and inflammation in the ovarian IR model and promoted ovarian folliculogenesis. Keywords: Ovary, Ischemia/reperfusion, G-CSF, Azan, IL-6, Immunohistochemistry