Active Suppression Of Endopg Iv By Ligation Of The Pro-Sequence From Stereum Purpureum Pro-Endopg I

We attempted to inactivate endopolygaolacturonase from Stereum purpureum (EndoPG) IV of identical origin by linking the pro-sequence of S. purpureum ProEndoPG I to the C-terminus. The recombinant ProEndoPG IV, expressed in Escherichia coli, had no polygalacturonase (PG) activity, but activity was acquired after partial degradation of the pro-sequence with V8 protease, as was the case for Pro-EndoPG I. These results indicate that the pro-sequence of ProEndoPG I can suppress the PG activity of EndoPG IV.