Receptor For Activated C-Kinase (Rack-1), A Wd Motif-Containing Protein, Specifically Associates With The Human Type Iifn Receptor
Journal of immunology (Baltimore, Md. : 1950)(2000)
Abstract
The cytoplasmic domain of the human type I IFN receptor chain 2 (IFNAR2c or IFN-alphaR betaL) was used as bait in a yeast two-hybrid system to identify novel proteins interacting with this region of the receptor. We report here a specific interaction between the cytoplasmic domain of IFN-alphaR betaL and a previously identified protein, RACK-1 (receptor for activated C kinase), Using GST fusion proteins encoding different regions of the cytoplasmic domain of IFN-alphaR betaL, the minimum site for RACK-1 binding was mapped to aa 300-346, RACK-1 binding to IFN-alphaR betaL did not require the first 91 aa of RACK-1, which includes two WD domains, WD1 and WD2. The interaction between RACK-1 and IFN-alphaR beta, but not the human IFN receptor chain 1 (IFNAR1 or IFN-alphaR alpha), was also detected in human Daudi cells by coimmunoprecipitation. RACK-1 was shown to be constitutively associated with IFN-alphaR betaL, and this association was not effected by stimulation of Daudi cells with type I IFNs (IFN-beta 1b). RACK-1 itself did not become tyrosine phosphorylated upon stimulation of Daudi cells with IFN-beta 1b, However, stimulation of cells with either IFN-beta 1b or PMA did result in an increase in detectable immunofluorescence and intracellular redistribution of RACK-1.
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Key words
yeast two hybrid system,fusion protein
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