Inhibitory effect of anti-Lewis y antibody on alpha1,2-fucosyltransferase gene transfected human ovarian cancer cells in vitro]

AIM:To study the inhibitory effect of anti-Lewis y monoclonal antibody on proliferation,adhesion and invasion of α1,2-fucosyltransferase gene transfected human ovarian cancer cell line RMG-I-H with over-expression of Lewis y antigen in vitro.METHODS:The changes of proliferation,adhesion and invasion of RMG-I-H cells in vitro were detected by cell growth assay,cell adhesive assay and cell invasive assay,respectively,with goat anti-human IgG antibody treated group or non-antibody treated group as control.RESULTS:The proliferation and adhesion of RMG-I-H cells were remarkably inhibited by preincubation of the tumor cells with anti-Lewis y antibody in vitro(P<0.05).The growth inhibitory rate from the second day to the seventh day was 5.62%,19.75%,34.96%,46.51%,49.78% and 33.33%,respectively.The adhesion inhibitory rate at different time points(15,30,60 min)was 47.59%,58.64% and 13.85%,respectively.Invasive assay with a transwell cell culture chamber showed that there were no significant differences of invasion in vitro between each concentration of the Lewis y antibody treated group and non-antibody treated group(P>0.05).CONCLUSION:Anti-Lewis y antibody significantly inhibits the proliferation and adhesion of RMG-I-H cells cultured in vitro,but it has no effect on invasion,which indicates that Lewis y is associated with some biological behaviors,such as proliferation and adhesion.The antibody against Lewis y may be an effective anti-metastasis agent for the treatment of patients with ovarian cancer.