Inhibitory effect of polysaccharides on the six-alpha-helix bundle formation of HIV gp41 protein]

Objective To compare the in vitro inhibitory effect of expolysaccharides from Streptomyces,polysaccharides of Ganoderma lucidum and rice bran on six-α-helix bundle formation of HIV gp41 protein.Methods The amount of six-α-helix bundle formed in the presence of N36 and C34 was tested by ELISA in response to treatments with different doses of polysaccharides.Results Expolysaccharides from Streptomyces potentially inhibited six-α-helix bundle formation with the effective concentration(IC50)of 145.48±7.25 mg/L.Polysaccharides of Ganoderma lucidum and rice bran showed no effect on the six-α-helix bundle formation.Conclusions Expolysaccharides from Streptomyces can inhibit the six-α-helix bundle formation of HIV gp41,whereas polysaccharides of Ganoderma lucidum and rice bran do not exhibit such activity.