A combination of in vivo selection and cell sorting for the identification of enantioselective biocatalysts.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION(2011)
Abstract
The “carrot and stick” principle could be combined with cell sorting to enable the selection of enantioselective esterase variants from a mutant library. Hence, the enormous diversity generated in directed evolution experiments is now easily accessible by this high-throughput system. In line with the principle, the hydrolysis of 1 glycerin supports cell growth, whereas the hydrolysis of 2 leads to cell death.
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Key words
biocatalysis,directed evolution,enantioselectivity,flow cytometry,in vivo selection
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