Analysis of K. pneumoniae by Monoclonal Antibody: Immunohistochemical Detection of K. pneumoniae Surface Antigen Injected into Mice and Rats

The monoclonal antibody Kp62 recognized surface antigenic determinants of some strains of Klebsiella pneumoniae. The antigen recognized by Kp62 was demonstrated on the bacterial surface using immunoelectron microscopy. Kp62 reacted with K. pneumoniae No. 1 or K.pneumoniae B 5055 and lipopolysaccharide (LPS) from the same bacteria. However, Kp62 was not inhibited by the LPS from Escherichia coli (E. coli) 0111 :B4 and E. coli 055:BS. Thus, Kp62 might be a useful monoclonal antibody to detect K. pneumoniae and LPS from K. pneumomae.