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A Centrifugation-Free High-Throughput Protein Purification System Using In-Line Microfluidization

Denis Alenkin,Laila Yermekbayeva,Shariq Mujib, Anders Vesterberg,Elena Newman, Ken Yamazaki,Doug Cossar,Sirano Dhe-Paganon

PROTEIN EXPRESSION AND PURIFICATION（2011）

Cited 5|Views7
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Abstract
The majority of current high-throughput protein purification protocols include rate-limiting centrifugation steps. A column and nozzle assembly was developed that can be used in-line with microfluidization for the purification of bacterially-overexpressed, His-tagged proteins directly from bacterial cultures. Yields and purity are comparable with standard protocols. This large-scale protein purification protocol is easy to use and widely-applicable.
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Key words
Protein purification,His-tagged,IMAC microfluidizer,Silica resin,HisLink bead,In-line microfluidization
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