Supplemental Figure 5 from Evaluating Robustness and Sensitivity of the NanoString Technologies nCounter Platform to Enable Multiplexed Gene Expression Analysis of Clinical Samples

Supplemental Figure 5A: Correlation scatter plot of normalised log2 values illustrating stability of a CodeSet after expiry date. A CodeSet consisting of 30 genes including 4 housekeeping genes was tested with a set of 6 identical samples (5 Fresh DLBCL tissues and 1 DLBCL cell line HBL-1) over an extended time-period of 2.5 years; the second test was performed 1.5 years after the CodeSet expiry date. Supplemental Figure 5B: Correlation scatter plot of mean normalised log2 counts of triplicate UHR samples tested with a CodeSet consisting of 463 genes including 18 housekeeping genes subjected to 4 freeze/thaw cycles.