Far-field nanoscopy with reversible chemical reactions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION(2011)
Abstract
Revolution in resolution: Abbe's resolution limit has been overcome in fluorescence microscopy by using light-driven processes to switch the emission of fluorophores on and off. Alternatively, chemical reactions can be used, for example the coordination of Cu2+ ions to a fluorescent probe for the stochastic switching between spectroscopic states.
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Key words
chemical switches,fluorescent probes,localization microscopy,resolution limits,single-molecule spectroscopy
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