Localization, developmental regulation and stress induction of p8-L1 and p8-L2 in zebrafish

The transcription cofactor, p8 , has a basic helix-loop-helix motif and is involved in several metabolic processes. It has previously been reported that p8 plays an important role in stress response in zebrafish. We expanded on these studies to further investigate the roles of two p8- like cDNAs found in zerbrafish, p8-L1 and p8-L2 . Zebrafish p8-L1 and p8-L2 cDNAs consist of 742 bp and 813 bp, respectively. Both have the same open reading frame despite different genomic structures and encode a 76 amino acid polypeptide with conserved a Phospho_p8 domain. These two cDNA’s were abundant in different tissues in the zebrafish: p8-L1 was high in intestine and muscle tissue and in low abundance in the backbone, whereas the highest amount of p8-L2 was found in the backbone, similar to p8 . During embryogenesis, both p8-L1 and p8-L2 were abundant at the cleavage stage and decreased gradually in abundance from blastula to pharyngula stage. However, p8-L1 abundance increased during hatching as observed in p8 . Quantitative real-time PCR assay suggested that p8-L1 and p8-L2 were both up-regulated significantly under osmotic pressure and pH value challenges, suggesting an important role of p8-L1 and p8-L2 genes in stress response.