Preparation and Microinjection of x-Rhodamine-Labeled Vimentin.
CSH protocols(2007)
摘要
INTRODUCTIONIntermediate filaments (IF) are major cytoskeletal systems of vertebrate and many nonvertebrate cells whose expression is cell-type specific and developmentally regulated. This protocol describes the x-rhodamine labeling of one type of IF, vimentin, and a method for microinjection of the labeled vimentin into cultured cells. IF dynamics can then be examined with fluorescence microscopy.
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