Prostate-derived Sterile 20-like Kinase 1-α Induces Apoptosis: JNK- AND CASPASE-DEPENDENT NUCLEAR LOCALIZATION IS A REQUIREMENT FOR MEMBRANE BLEBBING
Journal of Biological Chemistry(2007)
摘要
We have demonstrated previously that full-length prostate-derived sterile 20-like kinase 1-alpha (PSK1-alpha) binds to microtubules via its C terminus and regulates their organization and stability independently of its catalytic activity. Here we have shown that apoptotic and microtubule-disrupting agents promote catalytic activation, C-terminal cleavage, and nuclear translocation of endogenous phosphoserine 181 PSK1-a and activated N-terminal PSK1-alpha-induced apoptosis. PSK1-a, unlike its novel isoform PSK1-beta, stimulated the c-jun N-terminal kinase (JNK) pathway, and the nucleajr localization of PSK1-alpha and its induction of cell contraction, membrane blebbing, and apoptotic body formation were dependent on JNK activity. PSK1-alpha was also a caspase substrate, and the broad spectrum caspase inhibitor benzyloxycarbonyl-VAD-fluoromethyl ketone or mutation of a putative caspase recognition motif ((916)DPGD(919)) blocked nuclear localization of PSK1-a and its induction of membrane blebs. Additional inhibition of caspase 9 was needed to prevent cell contraction. PSK1-a is therefore a bifunctional kinase that associates with microtubules, and JNK-and caspase-mediated removal of its C-terminal microtubule-binding domain permits nuclear translocation of the N-terminal region of PSK1-alpha and its induction of apoptosis.
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关键词
protein kinase,focal adhesion kinase,scaffold proteins,sterilization,cleavage,nuclear localization,phosphorylation
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