The Crystal Structure Of Sdr-Type Pyridoxal 4-Dehydrogenase Of Mesorhizobium Loti

Huy Nhat Chu,Jun Kobayashi,Bunzo Mikami,Toshiharu Yagi

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY（2011）

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摘要

Pyridoxal 4-dehydrogenase catalyzes the irreversible oxidation of pyridoxal to 4-pyridoxolactone and is involved in degradation pathway I of pyridoxine, a vitamin B-6 compound. Its crystal structure was elucidated for the first time. Molecular replacement with (S)-1-phenylthanol dehydrogenase (PDB code 2EW8) was adopted to determine the tertiary structure of the NAD(+)-bound enzyme.

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关键词

short-chain dehydrogenase/reductase, 4-pyridoxolactone, vitamin B-6, pyridoxine-degradation, symbiotic bacterium

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