Effect of genistein on p90RSK phosphorylation and cell proliferation in T47D breast cancer cells.
ANTICANCER RESEARCH(2011)
摘要
Background: The molecular mechanisms of genistein's proliferative effects on breast cancer cells are largely unknown. This study aimed to examine estrogen-receptor (ER)-related signaling molecules involved in genistein-associated cell proliferation and survival (ERK1/2, p90RSK, JNK, Akt and NFkB) and to correlate these results to cell proliferation. Materials and Methods: The effect of genistein on cell-signaling molecules was determined in T47D breast cancer cells by a Bioplex phosphoprotein detection kit. These results were confirmed by Western blotting and were correlated to cell proliferation by MTT assay. Results: Low and high concentrations of genistein induced an ERK1/2-independent decrease in phosphorylated p90RSK. This effect was accompanied by decreased cell proliferation at high concentrations and an increased response at low concentrations of genistein following a 48-hour exposure. Conclusion.: Concentration-dependent actions of genistein in T47D cells may be due to differential activation of signaling molecules.
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关键词
Genistein,breast cancer,p90RSK,cell proliferation,T47D cells
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