Oligonucleotide labeling using BODIPY phosphoramidite.

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS（2011）

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摘要

4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) derivatives were prepared and their photochemical properties were characterized. One such analogue, 4,4-difluoro-4-bora-1,3,5,7-tetramethyl-8-(5-hydroxypentyl)-3a,4a-diaza-s-indacene was transformed into the corresponding phosphoramidite and incorporated into oligodeoxyribonucleotides as a fluorescent reporter group.

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关键词

BODIPY,fluorescent labeling,oligonucleotide,solid-phase synthesis,phosphoramidite

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