Interspecific Polymorphism of Gene lipL32 Restriction Profiles of Pathogenic Leptospires

We analyzed restriction fragment length polymorphism of lipL32 gene encoding outer membrane protein in three leprospira genomic species ( L. interrogans, L. kirschneri, and L. borgpetersenii ) using Bsa29 I and Bam HI restriction endonucleases. It was found that restriction profiles of the studied gene were similar at both the intraspecific and serogroup levels; variability was revealed only at the interspecific level, which can be explained by relatively low variability of lipL32 gene.