Effect of NGF on the Subcellular Localization of Group IIA Secretory Phospholipase A 2 (GIIA) in PC12 Cells: Role in Neuritogenesis
Neurochemical Research(2010)
Abstract
Phospholipases A 2 (PLA 2 s) are involved in neuritogenesis but the identity of the isoforms(s) contributing to this process is still not defined. Several reports have focused on secretory PLA 2 s (sPLA 2 ) as the administration of exogenous sPLA 2 s to PC12 neuronal cells stimulates neurite outgrowth. The present study demonstrates that the endogenous group IIA sPLA 2 (GIIA), constitutively expressed in mammalian neural cells, changes its subcellular localization when PC12 cells are induced to differentiate by NGF treatment. Indeed, confocal analysis showed a time-dependent accumulation of GIIA in growth cones and neurite tips. Under identical conditions the subcellular distribution of another isoform (GV) was unaffected by NGF. Contrary to GX, another sPLA 2 isoform expressed by PC12 cells, the contribution of GIIA to neuritogenesis does not require its release in the extracellular medium.
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Key words
Secretory phospholipase A2,PC12 cells,NGF,Neuritogenesis
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