Glucose and Galactose Transport in Bifidobacterium bifidum DSM 20082
Current microbiology(1997)
Abstract
. Sugar uptake was measured with 3 H-galactose and 14 C-glucose. Galactose transport system was not modified by inhibitors of known translocases and did not present a saturation kinetic with high concentration of galactose. Glucose incorporation was inhibited by lasalocid (cation symport inhibitor) and increased by KCl. The kinetic parameters K M and V max were respectively 9.16 mM and 26.56 nmol/min/mg cell protein. On the basis of this study, galactose crossed through the membrane by diffusion, and glucose was incorporated by a cation symport which is regulated by K + ions.
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Key words
Glucose,Sugar,Kinetic Parameter,Galactose,Transport System
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