A quantitative targeted proteomics approach to validate predicted microRNA targets in C. elegans

Marko Jovanovic,Lukas Reiter,Paola Picotti,Vinzenz Lange,Erica Bogan,Benjamin A Hurschler,Cherie Blenkiron,Nicolas J Lehrbach,Xavier C Ding,Manuel Weiss,Sabine P Schrimpf,Eric A Miska,Helge Großhans,Ruedi Aebersold,Michael O Hengartner

Nature Methods（2010）

Cited 74|Views30

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Abstract

MicroRNA targets predicted by a variety of computational tools can be validated using a quantitative targeted proteomics approach, using stable isotope labeling and selected reaction monitoring mass spectrometry. The authors used this method to confirm predicted let-7 and miR-58 targets in Caenorhabditis elegans .

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