Effect Of Cell Proliferation On Initiation Of Aflatoxin B-1-Induced Enzyme Altered Hepatic Foci In Rats And Hamsters

Rat is susceptible whereas hamster is resistant to aflatoxin B-1 (AFB(1)) hepatocarcinogenesis. Effect of cell proliferation on AFB(1)-induced glutathione S-transferase placental form (GST-P) positive foci has been examined in these two species after a single i.p. dose of AFB(1) and phenobarbital (PB) as a promoter in a 3 week period. Bromodeoxyuridine incorporation as a measure of cell. proliferation and GST-P hepatic foci were analyzed by immunohistochemical methods, Hepatic cell proliferation was maximum at 24 h after either partial hepatectomy (PH) or CCl4 (4 mmol/kg) pretreatment of rats whereas cell proliferation was maximum at 48 h after PH or CCl4 (1 mmol/kg) treatment of hamsters, Enhanced number of GST-P positive hepatic minifoci (two to nine cells) and foci (>100 mu) and focal area were observed in rats with either AFB(1) (0.5 mg/kg) given 24 h after PH or AFB(1) (0.5 or 2.5 mg/kg) given 48 h after CCl4 dosing, In hamsters, 1 or 2 mg AFB(1) treatment produced only GST-P positive single hepatocytes without presence of any minifoci whereas 3 or 6 mg AFB(1) produced minifoci consisting only of doublets. Pretreatment with CCl4 48 or 72 h before 1 mg AFB(1) dose level increased GST-P positive single cells and minifoci several fold, PH 24 or 48 h before 1 or 2 mg AFB(1) dose level increased minifoci, However, increase in minifoci was higher in PH hamsters at 48 h compared with those at 24 h, These results indicate that even though maximum initiation occurs in both speices when AFB(1) is administered at the peak of DNA synthesis, rats are more responsive than hamsters to cellular proliferation in the initiation phase of AFB(1)-induced hepatocarcinogenesis.