Effect Of Serum From Partially Hepatectomized Cirrhotic And Noncirrhotic Rats On Liver Regeneration With Primary Hepatocyte Cultures
EUROPEAN SURGICAL RESEARCH(1996)
Abstract
The effects of serum from cirrhotic and noncirrhotic rats after 33% and 70% partial hepatectomy on the hepatocyte culture were studied, Liver cirrhosis was produced by weekly intragastric feeding with carbon tetrachloride (CCl4) for 12-16 weeks. The serum was prepared with blood withdrawn from the rats 2 and 48 h after partial hepatectomy or sham operation. The incorporation of [H-3]thymidine in the hepatocyte culture after addition of rat serum (1.0 +/- 10(5)cells/ml) was assessed. We found that the mitogenic effect of the serum at 2 and 48 h after partial hepatectomy was not significant. In the 70% hepatectomy group, the serum from cirrhotic rats had a stronger stimulatory effect to induce hepatocytes in primary culture entering DNA synthesis than that from noncirrhotic rats; but the difference was not statistically significant. The mitogenic effect of the serum from noncirrhotic rats after 33% hepatectomy was significantly lower than that from cirrhotic rats after 33% hepatectomy (p < 0.05). In this series, the serum from the cirrhotic rats after 33% hepatectomy had the strongest mitogenic effect; the serum from the partially hepatectomized rats with cirrhotic and non-cirrhotic livers increased [H-3]thymidine uptake into hepatocyte DNA significantly more than control serum or plasma.
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Key words
hepatocyte growth factor,cirrhosis,liver regeneration,partial hepatectomy
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