Conformational dynamics of single pre-mRNA molecules during in vitro splicing

John Abelson,Mario Blanco,Mark A Ditzler,Franklin Fuller,Pavithra Aravamudhan, Mona Wood,Tommaso Villa,Daniel E Ryan,Jeffrey A Pleiss,Corina Maeder,Christine Guthrie,Nils G Walter

NATURE STRUCTURAL & MOLECULAR BIOLOGY（2010）

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摘要

The spliceosome is a complex small nuclear RNA–protein machine that removes introns from pre-mRNAs. A single-molecule fluorescence resonance emission transfer (FRET) assay using the efficiently spliced yeast pre-mRNA, Ubc4, reveals many reversible, time- and ATP-dependent changes in conformation for individual pre-mRNAs and indicates that spliceosome assembly occurs close to thermal equilibrium.

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nature structural and molecular biology, science

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