Effects of WIN 55, 212-2 on I K current in cultured trigeminal ganglion neurons of rat

Summary To investigate the effects of WIN 55, 212-2 on I K in cultured rat trigeminal ganglion (TG) neurons, whole-cell patch clamp techniques were used to record the I K before and after WIN 55,212-2 perfusion at different concentrations. 30 μmol/L WIN 55,212-2 markedly (35.7%±7.3% P <0.01, n =8) inhibited I K currents, and the currents were partially recovered after washing. 30 μmol/L WIN 55, 212-2 also induced a significant depolarizing shift in conductance-voltage parameters (control: V 0.5 =10.43±4.25 mV, k=16.27±3.86; WIN 55,212-2: V 0.5 =24.71±3.91 mV, k=16.69±2.75; n =8, P <0.01 for V 0.5 ). 0.01 μmol/L WIN 55,212-2 slightly (27.0%±7.9%, P <0.05, n =7) increased I K currents, but had no significant change in conductance-voltage parameters (control: V 0.5 =10.74±5.27 mV, k=17. 33±2.96; WIN 55, 212-2: V 0.5 =11.06±2.05 mV, k=19.69±6.60; n=7 , P >0.05 for V 0.5 and k). These results suggested that WIN 55, 212-2 has dual action, which might be through different receptors.