Visualization Of Ultraviolet Light-Induced Thymine Dimers In Dna By Immunoelectron Microscopy

Masako U. Udono,Makoto Hori, Michiko Hirayama, Hikotaro Yoshida, Tadashi Suematsu, Takashi Suematsu,Toshio Mori,Tsukasa Matsunaga, Terumi Mizuno,Osamu Nikaido

PHOTOCHEMISTRY AND PHOTOBIOLOGY（1993）

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摘要

To see the damage of DNA due to ultraviolet-B more distinctly, immunoelectron microscopic studies using a monoclonal antibody against cyclobutane-type thymine dimers were performed. As a result, we could detect the existence of thymine dimers on human genomic DNA and pUC18 plasmid DNA visually. This technique can be useful to locate the photoproducts formed on DNA.

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