HYNAPENE-A, HYNAPENE-B AND HYNAPENE-C, NEW ANTICOCCIDIAL AGENTS PRODUCED BY PENICILLIUM SP .1. PRODUCTION, ISOLATION AND PHYSICOCHEMICAL AND BIOLOGICAL PROPERTIES

Penicillium sp. FO-1611, a soil isolate, was found to produce a series of new anticoccidial compounds. Three active compounds, designated hynapenes A, B and C, were isolated from the fermetation broth of the producing strain by solvent extraction, silica gel column chromatography, gel filtration on Sephadex LH-20 and preparative HPLC. Hynapenes inhibited the growth of Eimeria tenella in an in vitro assay using BHK-21 cells as a host. No schizont in the cells was observed at concentrations ranging above 123 mu M, 34.7 mu M and 34.7 mu M for hynapenes A, B and C, respectively.