Reply To Sheward Et Al.: Two Sampling Issues In Using Primer Ids
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA（2012）
摘要
We thank Sheward et al. (1) for their letter regarding our article (2) and for reminding the reader of the importance of sampling depth when using unique sequence tags (Primer IDs) to determine the starting number of templates. A Primer ID reveals previously unseen biases and errors from PCR and deep sequencing protocols, especially when applied to highly heterogeneous viral …
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