Elucidation Of Synaptonemal Complex Organization By Super-Resolution Imaging With Isotropic Resolution
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA(2015)
摘要
Synaptonemal complexes (SCs) are meiosis-specific multiprotein complexes that are essential for synapsis, recombination, and segregation of homologous chromosomes, but the molecular organization of SCs remains unclear. We used immunofluorescence labeling in combination with super-resolution imaging and average position determination to investigate the molecular architecture of SCs. Combination of 2D super-resolution images recorded from different areas of the helical ladder-like structure allowed us to reconstruct the 3D molecular organization of the mammalian SC with isotropic resolution. The central element is composedoftwoparallel cables at a distance of similar to 100 nm, which are oriented perpendicular to two parallel cables of the lateral element arranged at a distance of similar to 220 nm. The two parallel cable elements form twisted helical structures that are connected by transversal filaments by their N and C termini. A single-cell preparation generates sufficient localizations to compile a 3D model of the SC with nanometer precision.
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关键词
super-resolution imaging, synaptonemal complex, dSTORM, meiosis, average position determination
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