Optically-induced dielectrophoretic technology for cancer cells identification and concentration.

The detection and concentration of cancer cells in peripheral blood is of great importance for cancer diagnosis and prognosis. Optically-induced dielectrophoresis (ODEP) can achieve high resolution and low optical intensities, and the electrodes pattern can be dynamically changed by varied light pattern. In this paper, a special lens is used to project the entire image to the ODEP chip to achieve 2.6 × 2 mm(2) manipulating area. By changing projected light pattern, it is demonstrated to separate 10, 20, and 40 µm PS (polystyrene) beads; HT-29, 20 µm PS beads. The MCF-7 cells concentrated experiments are also demo at 100 µm/sec velocity.