Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells

Amy Y Hsiao,Teru Okitsu,Hiroaki Onoe,M Kiyosawa,Hiroki Teramae,Sadaaki Iwanaga, Seiichi Miura,Tomohiko Kazama,T Matsumoto,Shoji Takeuchi

Micro Electro Mechanical Systems（2013）

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摘要

This paper describes the self-assembly of “cell springs” using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.

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关键词

biomems,bioelectric phenomena,cellular biophysics,microfabrication,microfluidics,mixtures,molecular biophysics,muscle,proteins,self-assembly,3d fiber-shaped microconstruction,dfat cell fibers,cell springs,coiled spring structures,collagen,differentiation induction,electrical stimulation,extracellular matrix mixture,fibrin,microfluidic coaxial device,multipotent dedifferentiated fat cells,smooth muscle-like cell differentiation,synchronized contraction,tubular alginate microfibers,self assembly

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