5-azacytidine induces chromosome fragmentation in the root tips of wheat carrying the gametocidal chromosome 2C
BMEI), 2010 3rd International Conference(2010)
Abstract
The gametocidal chromosome 2C from Aegilops cylindrica was used to analyze the relationship between methylation and gametocidal action. The root tips of wheat carrying the gametocidal chromosome 2C were treated by the hypomethylation agent 5-azacytine (5-AC) in 50μM concentration. The chromosome breakages and micronucleus were observed in these root tips. The results suggest that 5-AC treatment specifically induced chromosome breakage, furthermore, the DNA methylation protected the fragmentation-inhibitor function or suppress the fragmentation-inducer function of gametocidal chromosome 2C, so that repress chromosome breakage induced by the Gc factors during early seed development.
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Key words
botany,cellular biophysics,organic compounds,5-azacytidine,aegilops cylindrica,chromosome fragmentation,gametocidal action,gametocidal chromosome 2c,methylation,micronucleus,root tip,wheat,dna methylation,gametocidal chromosomes,genomics,bioinformatics,dna
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