A RNomics-based strategy identifies regulatory small RNAs in Burkholderia cepacia complex

Christian G Ramos,Silvia A Sousa,Andre M Grilo,Paulo J P Da Costa,Joana R Feliciano,Jorge H Leitao

Bioengineering（2011）

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摘要

Regulatory small non-coding RNAs (sRNAs) are know to be involved in bacterial virulence, most often exerting its function in a Hfq-dependent fashion. Burkholderia cepacia complex (Bcc) bacteria are important human opportunistic pathogens, that possess exceptionally large and complex genomes (-7000 genes) comprising roughly 8 Mb. Within their genomes, it has been predicted in silico 213 sRNAs. Bcc are unique among human pathogens by encoding two distinct and functional Hfq-like proteins. In this work, we describe a strategy based on coimmunoprecipitation of sRNAs with Hfq-like proteins, the cloning and identification of the sRNAs, showing the wide array of possibilities that this RNomics approach endows the researcher for sRNA discovery.

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关键词

diseases,genetics,genomics,microorganisms,molecular biophysics,proteins,bcc bacteria,burkholderia cepacia complex,hfq-dependent fashion,hfq-like proteins,rnomics approach,rnomics-based strategy,bacterial virulence,complex genome,functional hfq-like proteins,gene,human opportunistic pathogens,in silico 213 srna,regulatory small noncoding rna,srna cloning,srna coimmunoprecipitation,srna discovery,srna identification,coimmuniprecipitation,hfq,rnomics,srna,non coding rna,rna,bioinformatics,cloning

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