Fabrication and Packaging of Nanoporous Membrane Chips for Label-Free Ion-Channel Transducer Based Biosensor

Vincent Agache,Fabien Sauter,C Pudda,R Blanc,Claude Chabrol,Patrice Caillat,Thomas Plenat,A V Agasoster, L Ghenim,A M Fuchs

Lyon（2007）

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Abstract

This paper relates to a new architecture of label-free Ion channel based biosensors. The biochips are composed of nanoaperture arrays etched in a free-standing silicon nitride (Si3N4) supporting membrane. These membranes should be covered lately by a lipid bilayer which hosts bioengineered protein receptors-gated ion channels. This protein complex is used to convert specific binding events with target molecules into an electrical signal. This paper is focusing on the fabrication and packaging of the device.

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Key words

biomembrane transport,biosensors,membranes,nanoporous materials,proteins,silicon compounds,bioengineered protein receptors-gated ion channels,biosensor,free-standing silicon nitride,label- free ion-channel transducer,lipid bilayer,nanoaperture arrays,nanoporous membrane chips,specific binding events,nanoporous membrane chip,on channel based biosensors,chip,fabrication,lipidomics,packaging,silicon,etching,ion channel,transducers,protein complex

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