Two-photon fluorescence resonance energy transfer stoichiometry in living cells

Amar R Bhagwat,Daniel C Flynn,Meredith H Brenner,Marcos Nunez,Jennifer P Ogilvie,Dawen Cai,Joel A Swanson

Lasers and Electro-Optics（2014）

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摘要

Using phase-shaped pulses, we perform, for the first time, a proof-of-principle demonstration of two-photon fluorescence resonance energy transfer (FRET) microscopy for studying the stoichiometry of intermolecular interactions within living cells.

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关键词

cellular biophysics,fluorescence,molecular biophysics,optical microscopy,stoichiometry,two-photon processes,fret microscopy,intermolecular interactions,living cells,phase-shaped pulses,proof-of-principle demonstration,two-photon fluorescence resonance energy transfer microscopy,pulse shaping

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