The versatile electron microscope: an ultrastructural overview of autophagy.
Methods(2015)
摘要
•Block-face imaging techniques yield three-dimensional data of relatively large volumes with ultrastructural resolution.•Dual-axis electron tomography resolves nano-structural detail of smaller volumes.•CLEM and immunoEM assist in ultrastructural localization of proteins.•High-pressure freezing followed by freeze substitution yields excellent ultrastructural preservation.
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关键词
Autophagy,Cryofixation,Electron tomography,ImmunoEM,Volume EM,Correlative light-electron microscopy
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