CMV specific cytokine release assay in whole blood is optimized by combining synthetic CMV peptides and toll like receptor agonists.

Werner Dammermann,David Bochmann,Frank Bentzien,Lars Komorowski,Katja Steinhagen,Sebastian Ullrich,Jan van Lunzen,Stefan Lüth

Journal of Immunological Methods（2014）

引用 12|浏览12

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摘要

•We developed a CMV specific IGRA comparing synthetic CMV peptides, CMV native lysate and CMV recombinant antigen.•TLR agonists were tested to enhance CMV antigen immunogenicity.•Synthetic CMV peptides or native lysate showed the best cytokine signal to noise ratio compared to CMV recombinant antigen.•TLR agonists LTA and Poly(I:C) constitute potential costimulating reagents for CMV specific IGRAs.

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关键词

Cytomegalovirus,CMV,Toll like receptor,Agonist,Interferon gamma release assay

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