Transcriptome Analysis Of Rhizoctonia Solani Anastomosis Group 5 Early Invasion In Potato

Rhizoctonia solani (R. solani) is a destructive soil-borne root infecting fungus which impaired quality and production of potato. This study was performed to investigate the response to R. solani AG-5 infection in potato. Minitubers (cv. Favorita) were inoculated by the R. solani AG-5 strain. Four days after emergence of R. solani AG-5 infection on the young stem of potato, samples were collected and prepared for the Illumina sequencing. The Gene Ontology (GO) and KEGG pathway enrichment analyses of differentially expressed genes (DEGs) between infected and control plants were performed. Functional analysis of these DEGs indicated a total of 338 DEGs, including 216 up-regulated (63.91%) and 122 downregulated DEGs (36.09%), were identified between the control and R. solani infected potato plants. These DEGs were associated with the plant growth, defense, fungal resistance by modulating the oxidative response (including NB-ARC domain-containing disease resistance proteins, ankyrin repeat-containing proteins, ANKs; and peroxidases), ion exchange/transportation (including calcineurin B-like proteins, CBLs; CBL-interacting protein kinase, CIPKs; calmodulin-like proteins, CMLs; and K+ channel, KAT1) and plant hormone-mediated signaling (MYB44, ANKs). Some essential transcription factors including WRKY45 and MYB4 were significantly upregulated by R. solani infection. The R. solani infection triggered DEGs were related to plant growth, defense, oxidative response, and hormone-mediated signaling. The genes of CBL4, KAT1, ANKs, and MYBs might promote potato adjustment or resistance against R. solani infection. (C) 2018 Friends Science Publishers