Engineering a high-performance, metagenomic-derived novel xylanase with improved soluble protein yield and thermostability
Enzyme and Microbial Technology（2015）
Abstract
•A novel metagenomic-derived xylanase gene xyl7 was isolated and expressed.•The enzyme XYL7 showed higher specific activity and broad pH activity range.•A truncated XYL7-Tr3 with tenfold increase in soluble protein yield was acquired.•Mutants showed 250-fold and 10°C increase in half-life and optimal temperature.•The reducing sugar produced from xylan by mutant XYL7-TC was increased fourfold.
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Key words
Directed molecular evolution,GH11 xylanase,Metagenomic,Protein engineering,Thermostability
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