Application of coupled affinity-sizing chromatography for the detection of proteolyzed HSA-tagged proteins.
Protein Expression and Purification（2015）
Abstract
•Analytical technique (ALC–SEC) successfully detected protein clipping in cell culture.•Addition of protease inhibitor reduced the proteolysis.•In-culture results correlated strongly with purified downstream analytics.
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Key words
Purification,HPLC,Gel filtration chromatography,Protease inhibitors,Affinity chromatography
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