Bacillus dabaoshanensis sp. nov., a Cr(VI)-tolerant bacterium isolated from heavy-metal-contaminated soil

A Cr(VI)-tolerant, Gram-staining-positive, rod-shaped, endospore-forming and facultative anaerobic bacterium, designated as GSS04 T , was isolated from a heavy-metal-contaminated soil. Strain GSS04 T was Cr(VI)-tolerant with a minimum inhibitory concentration of 600 mg l −1 and was capable of reducing Cr(VI) under both aerobic and anaerobic conditions. Growth occurred with presence of 0–3 % (w/v) NaCl (optimum 1 %), at pH 5.5–10.0 (optimum pH 7.0) and 15–50 °C (optimum 30–37 °C). The main respiratory quinone was MK-7 and the major fatty acids were anteiso-C 15:0 and iso-C 15:0 . The DNA G+C content was 41.1 mol%. The predominant polar lipid was diphosphatidylglycerol. Based on 16S rRNA gene sequence similarity, the closest phylogenetic relative was Bacillus shackletonii DSM 18868 T (97.6 %). The DNA–DNA hybridization between GSS04 T and its closest relatives revealed low relatedness (<70 %). The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain GSS04 T represents a novel species of the genus Bacillus , for which the name Bacillus dabaoshanensis sp. nov. is proposed. The type strain is GSS04 T (=CCTCC AB 2013260 T = KCTC 33191 T ).