MRI观察金磁微粒结合抗podoplanin抗体体外标记淋巴管内皮细胞
Chinese Journal of Medical Imaging Technology(2011)
Abstract
目的 制备淋巴管内皮细胞(LEC)靶向分子探针(GoldMag-pod),探讨GoldMag-pod对LEC的体外标记情况及其在MR检查中对信号强度的影响.方法 将金磁微粒(GoldMag)与抗podoplanin抗体通过"一步法"耦联,制备GoldMag-pod,以分光光度计检测GoldMag的抗体耦联效率,ELISA法检测抗体与GoldMag结合前、后的免疫活性,免疫荧光染色、普鲁士蓝染色检测GoldMag-pod对LEC的标记情况.用不同浓度GoldMag-pod标记LEC后,观察GRE T2*WI信号强度的改变.结果 GoldMag与抗podoplanin抗体通过"一步法"耦联成功,GoldMag的抗体耦联效率为52.94%,抗podoplanin抗体与GoldMag结合后其免疫活力降低为结合前的42.05%;GoldMag-pod标记后的LEC发出特异性绿色荧光,标记阳性率随GoldMag-pod浓度的增高而增高.GoldMag-pod标记后的LEC悬液的GRE T2*WI信号强度明显降低.结论 GoldMag与抗podoplanin抗体耦联制备的分子探针GoldMag-pod具有较高免疫活性,能特异性标记LEC,产生MR靶向增强作用.
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Key words
Endothelial cells,Magnetic resonance imaging,Molecular imaging,Molecular probes,PDPN protein, human
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