A model for hypothermic perfusion of porcine kidney in vivo: Morphologic characteristics
Human Health and Biomedical Engineering(2011)
Abstract
In this study, we established a model of hypothermic perfusion for porcine kidney in vivo and investigated protective effect in intraoperative on porcine renal cells. 24 porcine kidneys were randomly divided into pseudo-operation group, hypothermic perfusion group and arterial occlusion group. We detected expression of Caspase-3 protein and ultrastructure in renal cells by immunohistochemistry and transmission electron microscope. Results showed the artery occlusion group could examine cell apoptosis, necrosis ever since 1 h and hypothermic perfusion group didn't change significantly. The artery occlusion group was higher than other groups on the apoptosis index and expression of Caspase-3 ever since 0.5 h. The nuclear and mitochondrial swelling in 0.5 h and nuclear pyknosis was observed in 1 h in artery occlusion groups. Cell swelling only appeared in 0.5 h, and cell nucleus and mitochondria swelling in 1 h in hypothermic perfusion group. Therefore, the model can be used to improve the quality of kidney protection in surgical operation in vivo.
MoreTranslated text
Key words
cell swelling,porcine renal cells,pseudooperation group,cellular biophysics,time 1 h,haemorheology,in-vivo surgical operation,blood vessels,protective effect,mitochondrial swelling,caspase-3 protein,renal surgery,enzymes,kidney protection,morphologic characteristics,biochemistry,in-vivo porcine kidney,molecular biophysics,cell apoptosis,cell nucleus,transmission electron microscopy,ultrastructure,kidney,arterial occlusion group,immunohistochemistry,empirical study,nuclear pyknosis,surgery,necrosis,hypothermic perfusion,transmission electron microscope
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined