High-Light Induced Superoxide Radical Formation In Cytochrome B(6)F Complex From Bryopsis Corticulans As Detected By Epr Spectroscopy

The generation of superoxide radical (O-2(center dot-)) in Cyt b(6)f of Bryopsis corticulans under high light illumination was studied using electron paramagnetic resonance (EPR) spectroscopy. This could be evidenced by the addition of SOD which specifically reacted with O-2(center dot-). The generation of O-2(center dot-) was lost in the absence of oxygen and was found to be suppressed in the presence of NaN3 and be scavenged by extraneous antioxidants such as ascorbate, beta-carotene and glutathione which could also scavenged O-1(2)center dot-. These results indicated that O-2(center dot-) which produced under high light illumination in Cyt b(6)f of B. corticulans might rise from a reaction which O-1(2)* could participated in. Also the photo-protection mechanism to Cyt b(6)f complex by antioxidants which might contain in thylakoid was speculated. (C) 2011 Published by Elsevier B.V.