Screening of xylitol-producing strain and optimization of its fermentation conditions

A yeast, which can perform biotransformation from xylose to xylitol, was isolated from corncob and was identified as trichosporon coremiiforme by morphological identification and 26S rDNA D1/D2 domain sequence analysis. Besides, the optimal fermentation conditions were determined through single factor test and orthogonal experiment. The optimal conditions are as follows, carbon source concentration: 40 g/l, nitrogen source concentration: 6 g/l, initial pH: 7.0 and inoculum size: 6%.