Preliminary Study on Repeatability of Molecular Recognition Capability of a Silica-Based Molecularly Imprinted Polymer
Bioinformatics and Biomedical Engineering(2010)
Abstract
Molecular imprinting, a templated polymerization technique, is a promising method to prepare a functional polymeric network for targeted binding of molecules in a selective recognition process. In this work, a silica-based (polysiloxane) polymer was utilized in a sol-gel process to fabricate a molecularly imprinted polymer (MIP). The manufactured MIP was imprinted with a template molecule, lysozyme. To test this MIP's molecular recognitive function, the template molecule lysozyme was extracted first; then lysozyme and a competitive molecule, RNase A, were re-bound; then lysozyme and RNase A were released and re-bound for a second time in order to evaluate the recognition repeatability of the MIP. Analysis of the repeated testing results implies that the template molecules' conformation and affinity played a great role in affecting the recognition accuracy and efficiency.
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Key words
molecular recognition,template molecular conformation,bonds (chemical),sol-gel processing,polysiloxane,sol-gel process,targeted binding,polymerisation,polymers,templated polymerization technique,enzymes,molecularly imprinted polymer,lysozyme,biological techniques,molecular affinity,biochemistry,competitive molecule,molecular biophysics,molecular configurations,functional polymeric network,silicon compounds,silica-based molecularly imprinted polymer,molecular imprinting,silica,molecular recognition capability,rnase a,testing,sol gel process,proteins,scanning electron microscopy,protein engineering,biomedical engineering,fluorescence,fabrication
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