Multiplexed bead-based mesofluidic system for gene diagnosis and genotyping.

We have developed a novel multiplexed bead-based mesofluidic system (MBMS) based on the specific recognition events on the surface of a series of microbeads (diameter 250 pm) arranged in polydimethylsiloxane (PDMS) microchannels (diameter 300 pm) with the predetermined order and assembled an apparatus implementing automatically the high-throughput bead based assay and further demonstrated its feasibility and flexibility of gene diagnosis and genotyping, such as beta-thalassemia mutation detection and HLA-DQA genotyping The apparatus, consisting of bead based mesofluidic PDMS chip, liquid-processing module, and fluorescence detection module, can integrate the procedure of automated sampling, hybridization reactions, washing, and in situ fluorescence detection The results revealed that MBMS is fast, has high sensitivity, and can be automated to carry out parallel and multiplexed genotyping and has the potential to open up new routes to flexible, high-through put approaches for bioanalysis